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KMID : 0620920110430070401
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Experimental & Molecular Medicine
2011 Volume.43 No. 7 p.401 ~ p.410
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The role of promoter methylation in Epstein-Barr virus (EBV) microRNA expression in EBV-infected B cell lines
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Kim Do-Nyun
Song Yoon-Jae
Lee Suk-Kyeong
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Abstract
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Epstein-Barr virus (EBV) microRNAs (miRNAs) are expressed in EBV-associated tumors and cell lines, but the regulation mechanism of their expression is unclear yet. We investigated whether the expression of EBV miRNAs is epigenetically regulated in EBV-infected B cell lines. The expression of BART miRNAs was inversely related with the methylation level of the BART promoter at both steady-state and following 5-aza-2’-deoxycytidine treatment of the cells. The expression of BHRF1 miRNAs also became detectable with the demethylation of Cp/Wp in latency I EBV-infected cell lines. Furthermore, in vitro methylation of the BART and Cp promoters reduced the promoter- driven transactivation. In contrast, tricostatin A had little effect on the expression of EBV miRNA expression as well as on the BART and Cp/Wp promoters. Our results suggest that promoter methylation, but not histone acetylation, plays a role in regulation of the EBV miRNA expression in EBV-infected B cell lines.
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KEYWORD
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decitabine, DNA methylation, Herpesvirus 4, human, microRNAs, promoter regions, genetic
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